Hi there, I am new to using prism and am trying to plot some gene expression data but I cannot get it to show my error bars on any negative (ie down-regulated) values. I have manually entered the values I want plotting and using xy table format. Does anyone know what I am doing wrong? Many, many thanks!

Hey Pennatula,

Try going into 'Format Graph' (found in the 'Change' box at the top of the screen, or by double-clicking a data point on the graph) and click on the 'All' button on the top right corner of the 'Appearance' tab - this should change the 'Data set' selection to '---Change ALL data sets---'. Now any changes you make should be applied to all data sets.

Next try selecting 'Show error bars'. If the box is already ticked then un-select it and re-select it just to make doubly sure (sometimes GraphPad needs reminding). You can also select the direction of the error bars here.

Let me know if this works. Goodluck!

Hi Baseline, Thanks heaps for your reply - problem solved!