i used Ficoll-paque, i saw a pellet after i'd collected white layer, but soemthing went wrong after that i think, froze them slowly in FCS with 20%DMSO, more advice would be greatly appreciated

anyone separated lymphocytes from blood? thought i'd done everything properly then came to defrost them from nitrogen... and gone!!! any ideas?

i keep lights on still, hate dark corners

just started jen about 6 months

you should reconsider applying there, it has an excellent RAE rating, also there's a new studentship advertised in the school of tropical medicine, have alook at it, Dr Gordon is extremely friendly as are most of the staff there

in what area? my first degree was in physiology but did a masters too so i think that helped my application

ok, ordered 5000 of them from camlab, thanx for the help x

liverpool, and you?

immunology, looking at the immune response in muscle, so soon will be using fluorophores

thanx mathew

probably would be much easier to find

anyone know were i can order black eppendorfs from?

anytime

Hey laney feel free to chat to me

i started my ph.d 4 months ago and although i'm a very sociable person, i have only recently felt i'd been ' fully accepted' especially by the post-docs who seem to spend most of their time talking about other people in the lab, how did everyone else find working with new people? were they helpful or did they enjoy your despair?