Overview of SonjaB

Recent Posts

Phd or industry?
S

Hey guys,

I am a current Master student from Germany studying Neuroscience and I have to apply soon for a position for my master thesis.

I am still very unsure what the next steps will be after I finished my masters. Phd would be one option although I know for sure that I don't want to stay in academia, but rather would go to industry. Also I have the feeling that I finally want start working, since I am already 26 and I am really tired of moving from one place to another. I'm really looking forward to settle somewhere start a new life in a company.

I found a small company close to my hometown, where they offer a trainee program. I thought this might be something for me, since I will live close to my family and can start in a company. However I have no experience with that and I am not sure if such a trainee program is worth doing or if it might be better to do phd and then look for a position in company.

What is you experience or opinion on that problem?

IC50 value problem in Graphpad prism
S

Hello guys,

I am struggling with my data analysis in graphpad prism and looking for some help of advanced people.

I did a cell viability assay and plotted my datas in graphpad prism to calculate the IC50 value.

The curves I got look very different from each other and the calculated IC50 value seems not to represent the real values.

I draw some lines in my graph to get an idea of the real IC50 value and compared it to the ones from the table, however for one of my curves it seems much lower as the true value.

When setting the top to the last value of my curve, then it gives me the real value, however when doing so, it also sets this top for the other curve, which gives me then wrong value for this curve.

Is there a way to contraint the top only for one curve?
If you need more information or want to see the curve to get an idea of the problem please text me and I can try to send a image of the graph.

Thank you so much for help