Oh yeah, Sunday night all night. Two (smallish) chapters to go. Don't think I'll get them finished tonight mind you!:p

Yep sounds like you've got it sorted.

you can get to 1 million/ml lots of ways. I'd take 0.131 ml (of well mixed cell culture) and make it up to 1 ml.

Final conc/Current conc = vol. So 1/7.62 = 0.131

The Atlantic coast in France. Seafood, Sea and Seafood. French campsites are ace and usually of a really good standard. I stayed at a lovely site near La Rochelle a couple of years ago played ping pong galore, hired a bike and must have covered 150+ km during the week as well as finding some time to kick back and relax.

Have fun where ever you may go.

It sounds like there is another me out there! But really, I've had exactly the same issues with my supervisor. I used to get blanked so often I started wondering if I was invisible or if my supervisor was deaf. My sup's attitude seems to have made the whole Ph.D. process a lot more difficult for everyone involved. I'm actually very reluctant to discuss science with the sup as I have no idea what mood they'll be in; the responses I can get are anything from utter disgust to feigned interest, and unfortunately the former more often than the latter. Lucky, I have a partner who listens when I vent off over this stuff and a lab mate who gets the same treatment, you should find someone who can just sit and nod for a while whilst you let rip. Want to keep your thoughts private, draw a face on an orange and shout at it.

But yes, like yourself I'm fed up with the lack of respect, support, and interest my sup shows in anything related to me. It certainly has been an eye opener for me. Currently, I'm just focussing on getting the thesis finished and getting a job - the future could hold anything, even a pleasant boss. Like Porgand says try to ignore the sup (I know, I know... the it's easier said than done - esp if you're doing a lab based science), we haven't got long to go!
Good luck with the writing.
;-)

Doing a Ph.D. is really frustrating, but if you've got a good, supportive, clued up supervisor that you get along with it's not too bad.

Have a good chat with your supervisor(s), find out what they expect from you and what previous Ph.D. students from that group have gone on to do. Also ask what direction they would like the research to go in, then you know where to start and gain their support.

Hi Niall,

I've got to agree with missspacey on this one, it is a risk you'll be taking. I'm currently completing my Ph.D. and looking for jobs. I think it's going to be a couple of months (without stipend) before i'm employed again.

If your heart is really set on doing a Ph.D., don't let me put you off. Stipends are tax free and you can top it up with money from teaching, so it's pretty comfortable while you're there.

Just a thought, can you move sideways in the industry into something more research based - probably be more rewarding...