Hey,

how long could you prepare qPCR (SybrGreen) plates in advance before actually running them in the machine?

The machine here in this lab is so busy and belongs another lab, so they have booked all the nice slots during the day and what's left over is fed to the rest...
So I was thinking of preparing a plate the day before in the evening so I can come in the next morning very early and just have to put the ready made plate in.

Any ideas/suggestions to this problematic?

Thanks.

this sounds perfectly acceptable! I often prepare plates in advance the day before and run them the next day eg afternoon, if kept at 4C this is fine!

Ok cool. So how many hours have you been storing them plates exactly? How far would you extend this?

Thanks very much ;)