funny n numbers

B

Hi,

I'm analysing a bit of data, and long story short i have slightly different n numbers for some of my conditions - 2 have 8 and the rest have five. I am adjusting for this, but is it okay to present if they aren't consistant?

Also with one western blot, I have 3 non applicable samples - they dont affect the mean as such but can affect the SEM due to how I'm calculating it. For example, I have an n of 5 westerns, and i calculate the SEM by Standard deviation/Square root of the number of repeats. So generally this is five, but with the 3 I have non applicable samples, should it be 4 or 5?

Thanks

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