Hi, has anyone got a good protocol for using SYBR green in real time PCR???

try www.protocol-online.org

full of useful information!

No super-duper protocol in general (sorry), but I had a lot of problems with SYBR Green to start with. In general, I followed the ABgene protocol. Also, watch your primer concentrations and starting cncetration of cDNA or DNA. They are both key in generating good amplicons in SYBR green analysis.

http://www.abgene.com/productDetails.asp?prodID=31 there are some good data sheets here to download and potentially provide better real time results

hope it's of some help

G

Thanks! I'll see how I get on with those.