single cell analysis

posted
25-Sep-11, 20:09
by Ruth84
Avatar for Ruth84
posted about 9 years ago
hi guys,
what techniques can I apply working on a sigle cell?just one?
posted
26-Sep-11, 09:50
by Cakeman
Avatar for Cakeman
posted about 9 years ago
One cell, Why would you?

Do you mean you have several isolated cells, or simply one cell in a dish/on a coverslip.

My first piece of advice might be: can you produce more cells, as most cells don't survive very well entirely on their own

Failing that, you have some techniques available to you still. You could image it (load with fluorescent indicator dye), and you could immunostain it. I don't think PCR or anything that involves extractions would be possible with a cell (so no western blotting, RNA analysis etc).

If you need more info on live cell imaging or immunostaining then just ask

All the best, Cakeman
posted
26-Sep-11, 11:57
edited about 28 seconds later
by Ruth84
Avatar for Ruth84
posted about 9 years ago
Thanks Cakeman,
yes,
could you please give some more information?
posted
26-Sep-11, 13:12
by Cakeman
Avatar for Cakeman
posted about 9 years ago
Hi Ruth84,

I can give you more information eg protocols and references on the techniques. If you want more specific advice, then maybe you could give me some idea what you are trying to do with your single cell? Although it should be said that these are fairly standard techniques, most biomedical science type journals contain descriptions of various sorts that you could probably read and follow.

If you don't want to disclose this information to everyone, then remember you can use the personal message option to contact me only.

ATB cakeman
posted
26-Sep-11, 13:17
by Claudia
Avatar for Claudia
posted about 9 years ago
Maybe you could do some electrophysiology on it :-)

I'm not sure I understand your question entirely. Why only one cell? :p

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