Hello,

just as the title say, I wanted to ask how much are you expected to know? I used quite a lot of techniques and I am quite nervous as to how much detail will I be expected to know.
Techniques such as Circular dichroism.

How much into detail do you need to know?

A lot. And I didn't know know all the answers. What's the mode of action of ampicillin? I've looked this up a thousand times and I still don't remember, I just chuck it in the media and it kills the non-transformed bacteria.

They can pick anything from what you have written about and grill you until you can't provide further answers.
I was absolutely grilled on a couple of theoretical sections and it was very challenging but I got through it. My examiner was keen to firstly identify the limit of my knowledge and then drill through beyond that to see how I thought through unfamiliar problems. It was a pretty brutal session.