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Signup date: 12 Feb 2010 at 1:45pm
Last login: 12 Feb 2010 at 1:45pm
Post count: 2
I am doing real time PCR experiments and I am having problems with reproducibility fo the results. I recently switched from Syber Green to Taqman and the first experiment was total rubbish- I am using delta-delta Ct method for calculation.
Any suggestion on how to calculate the results accurately? and how to optimise the cDNA concentration and conditions:-)
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