Welcome to the world's leading Postgraduate Forum
An active and supportive community.
Support and advice from your peers.
Your postgraduate questions answered.
Use your experience to help others.
Signup date: 10 Dec 2014 at 5:08pm
Last login: 26 Dec 2014 at 9:16pm
Post count: 1
Hey all,
I am working with 35S labeled Methionine and I prepared fMet-tRNAfMet using it. I want to use this initiator tRNA for testing translation initiation but the problem is that my lab has only the 14C radioctivity counting protocol. I made the initiation reaction and took the counts using this protocol anyway but I do not know how to determine the initiation efficiency from the dpms I have now.The counts seem to be really high and are in the range of 200000.
Any suggestions would be highly appreciated, thanks !
PostgraduateForum Is a trading name of FindAUniversity Ltd
FindAUniversity Ltd, 77 Sidney St, Sheffield, S1 4RG, UK. Tel +44 (0) 114 268 4940 Fax: +44 (0) 114 268 5766
An active and supportive community.
Support and advice from your peers.
Your postgraduate questions answered.
Use your experience to help others.
Enter your email address below to get started with your forum account
Enter your username below to login to your account
An email has been sent to your email account along with instructions on how to reset your password. If you do not recieve your email, or have any futher problems accessing your account, then please contact our customer support.
or continue as guest
To ensure all features on our website work properly, your computer, tablet or mobile needs to accept cookies. Our cookies don’t store your personal information, but provide us with anonymous information about use of the website and help us recognise you so we can offer you services more relevant to you. For more information please read our privacy policy
Agree Agree