I m funded. thats why he do not want to let me go. i was planning to talk to my director of pstgraduate school but they are good frens. He might side him.... i m still looking for alternative way out.... i wonder is it possible like i continue to carry out my all my experiement without letting him know bout the details and then by the time i finish what i should do then i just apply for the viva ? wondering any one did this before?

Dear Athos, thanks for your advice. the problem is he is a very good talker he always twist and turn things around to get what he wants. after two years of observation, i sincerely sound that he actually not interested into research but just to please our dean to get himself promoted to get more powerful. He involve very little in supervising and from the very begining i m the one who initiate and plan all the experiements ( and i know very well that is part of my job) he does not seems to be interested unless he needs the data for conference. and he use my data ( i made up the poster for him) and he submtted the abstract without my name in there. it is obvious that he is just want me to stay more to work more for him. I wonder who can i refer to and is it ok to say no to him? How do i make a clear cut off line about my project and how do i confirm i have got enough of data? Will it be worse if i say no to him because i m just worried he ll make my life worse. When i think back, from the very begining he already try to prevent me from talking to others about anything including my CO-supervisor!! Now is clear about his intention!! Now i have got no one to turn to and as a witnes of my progress through out this 2 years. HELP!! i realy cant concentrate on my work....

today i almost fainted when i heard my supervisor said i m only going to finish my experiements in year 2011 (total 4 and a half yrs of experiement). I have been working extremely hard since the very begining for my PhD ( and i have got tonnes of data now, i joined since august 2007) because i m planning to finish my experiement works within 2 and a half years then starts writting after that. this is how me and my supervisor deal initially. assigment that he had assigned to me initially are done. Now, he keep adding new works for me and it seems like a never ending list. seems like he was just lying to me and trying to hold me longer to work more for him!!! i m so frustruted!! i was working so hard and now even my body cant take it anymore. Now, the new tonnes of work that he has just assigned to me is triple of what our deal initially. Feel like really being exploited. What can i do and i feel so helpless. any one had this experience b4? please advice !

thanks lot for your reply. yea.. actually i asked around ppl in my lab already and not many staffs for me to refer to because i m working in a newly set up lab. sigh... i know it is not a hard thing but it seens so hard to get some sources over here. i even called to the supplier and asked but it is already more than a week after my second call yet no reply from them. anyway, thanks lot for your reply. so the conclusion is i can use NaOH to adjust the pH huh? =)

Hey guys out there, wondering any one of you had the experience in using HEPES buffer? i m having problem in making a HEPES buffer with pH 7.4. After i dissolved my HEPES in the deionised water, It gave me about pH 5.2. Some one has suggest to adjust the pH with either HCl or NaOH. However, Is not it buffer should resist the change of the pH ? any one please advice me on this ? pleaseeeeeee....=)

======= Date Modified 26 34 2009 10:34:18 =======
========== This thread had been moved ==========
============= Edited by a Moderator =============
Hey guys out there, wondering any one of you had the experience in using HEPES buffer? i m having problem in making a HEPES buffer with pH 7.4. After i dissolved my HEPES in the deionised water, It gave me about pH 5.2. Some one has suggest to adjust the pH with either HCl or NaOH. However, Is not it buffer should resist the change of the pH ? any one please advice me on this ? pleaseeeeeee....=)

Hey guys out there, wondering any one of you had the experience in using HEPES buffer? i m having problem in making a HEPES buffer with pH 7.4. After i dissolved my HEPES in the deionised water, It gave me about pH 5.2. Some one has suggest to adjust the pH with either HCl or NaOH. However, Is not it buffer should resist the change of the pH ? any one please advice me on this ? pleaseeeeeee....=)

======= Date Modified 26 35 2009 10:35:06 =======
========== This thread had been moved ==========
============= Edited by a Moderator =============
Hey guys out there, wondering any one of you had the experience in using HEPES buffer? i m having problem in making a HEPES buffer with pH 7.4. After i dissolved my HEPES in the deionised water, It gave me about pH 5.2. Some one has suggest to adjust the pH with either HCl or NaOH. However, Is not it buffer should resist the change of the pH ? any one please advice me on this ? pleaseeeeeee....=)

======= Date Modified 14 44 2009 14:44:23 =======
========== This thread had been moved ==========
============= Edited by a Moderator =============
guys out there. i know mytopic is a bit specific but please advice me if u had any experince before on this. i had spent 4 months working onthe encapsulation efficiency(EE). Anyone had any exeprince before on determining the encapsulation efficiency of lipid nanoparticles? i have tried out few methods already but is not very conclusive. i wonder usually how to determine the EE? please help........

hey guys out there!! urgently need ur help!! wondering does any one leave the water bath on over night at 70C? i know all the water ll evaporate off. worried bout the consequences. it takes me 2 hours to back to uni to off it and no one else i could get in touch with at this moment! anyone had this experience b4? please tell me i ll be fine... aiksss =(