immunohistochemistry looking at immune cells

Excellent references are a top priority I believe and also a good understanding of what it takes to undertake a phd, being humble (not being over confident), and having the personality to fit into the research group (how would you fit in and work alongside the others)

I cant understand why they havent offered you one then, especially if you've completed a masters, I'd say you have enough experience with both an honours lab project and masterslab project!!
maybe try a research assistant post in the meantime, you may be offered one 'in house' then. i think some phds arent even advertised but already taken by people working in the department.

I tend to be in at 9:30 and leave at 5:30, in the beginning i took work home to read over the evening but i've stopped that now as i found i was burning myself out.

is it a science studentship you're applying for? If so then they are very competitive and usually require a masters nowadays along with a a 2:1 or above

this thread still alive?

they are snap frozen in OCT and isopentane. wrapped in foil then kept at -80 until needed. i'm just not sure after cutting sections onto slides whether to fix in acetone before storing or just air dry then freeze

some advice needed, i'm cutting all my frozen muscle samples and need to know whether to fix them in alcohol then freeze at -80 or just freeze them unfixed.....:-)

ULUG you are right. Gordon Brown went to the University of Edinburgh at 16.

thanks Lis, will ask around department to see if we have this equipment

my clan will sit and starve till i come home to cook!!
i'm doing a science Ph.D so no afternoons of 'pinktrifle' time unfortunately. leave the house at 8 home at half six, 5 days a week

hi, i have two teenage boys, a husband who works away alot and have just started my Ph.D after doing an MSc so ican sympathise. i spend all my time either helping them with their homework or making an attempt with the domestic chores. Working on my project is an escape from all the hassle at home. i've decided to have a split personality, slave at home and student during the day..... seems to be working so far

oh and i didnt count them before freezing, i assumed there wouldnt be many anyhow

Hi Richmond,
After i've pelleted the cells i resuspend in FCS alone, then FCS + 10% DMSO. then i wrap in cotton wool in polystyrene box and freeze in minus 80. i move the vials to liquid nitrogen 3 days later. i'm isolating neutrophils from only 1 ml of mouse blood so hard to get good separation of lymphcytes with Ficoll-Paque. thanks everyone for the advice, looking forward to 'starting all over again Monday'.....

HiJen,i defrosted them quickly in water bath them pit straight in fresh medium,
i looked at lymphocytes i'd frozen a few months ago and i could see cells but only a few,i think its going to be better to use them as soon as isolated instead of freezing them, oh and i was freezing themin 20% DMSO? could that be the problem?